Improved profiling of polyamines using two-dimensional gas chromatography mass spectrometry

TALANTA

卷:199

Article

Yu, Zhanru¹  Huang, Honglei¹  Zhang, Hanlin²  Kessler, Benedikt M.¹ 

[1] Univ Oxford, Nuffield Dept Med, Target Discovery Inst, TDI Mass Spectrometry Lab, Oxford OX3 7FZ, England

[2] Univ Oxford, Nuffield Dept Orthopaed Rheumatol & Musculoskelet, Kennedy Inst Rheumatol, Oxford 0X3 7FY, England

关键词: Polyamines;    Spermidine;    Spermine;    Putrescine 2-dimensional;    Gas chromatography;   

DOI  :  10.1016/j.talanta.2019.02.062

来源: Elsevier

【 摘 要 】

Polyamines are a class of poly-cationic aliphatic amines, playing a role in different cellular processes such as maintaining intracellular pH and membrane potential that are relevant for general cellular physiology and ageing. The development of analytical methods for detection and quantitation of this class of compounds has been challenging due to the basic nature of these species. Both liquid chromatography (LC) and gas chromatography (GC) have been applied for separation, mostly coupled to mass spectrometry (MS) for detection. However, current methodologies suffer from lengthy extraction protocols and limitations in separation and detection levels. Here, we present a simplified and optimised method for straightforward extraction of poly amine metabolites including spermine, spermidine, norspermidine, cadaverine and putrescine from cellular and tissue material. We demonstrate that strong acid-based extraction and chemical derivatisation not only improves isolation, but also recovery. Combined with two-dimensional gas chromatography, this method provides clear separation and femtomole sensitivity for the profiling of polyamines.

【 授权许可】

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