FEBS Letters | |
Glucose stimulates the biosynthesis of a human pancreatic islet cell protein detected by an antiserum against the human erythrocyte glucose transporter | |
Lernmark, Åke1  Bækkeskov, Steinunn1  | |
[1] Hagedorn Research Laboratory, Niels Steensensvej 6, DK-2820 Gentofte, Denmark | |
关键词: Human erythrocyte glucose transporter; Human pancreatic islet; Glucose transport; β-Cell protein; Glucose regulation; 3-Isobutyl-1-methylxanthine; NCS; newborn calf serum; HS; human serum; IBMX; 3-isobutyl-1-methylxanthine; FCS; foetal calf serum; HEPES; N-hydroxy-ethyl-piperazine-N-2-ethane-sulfonic acid; PMSF; phenylmethylsulfonyl-fluoride; PCMBS; p-chloromercuribenzenesulfonic acid; SDS; sodium dodecylsulfate; cAMP; cyclic adenosine 3′; 5′-monophosphate; | |
DOI : 10.1016/0014-5793(83)80570-5 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
An antiserum raised against the purified human erythrocyte glucose transporter specifically immunoprecipitated a major protein of M r36 000 and a minor protein of M r 58 000 from human pancreatic islets. The biosynthetic incorporation of [35S]methionine into the 36-kDa protein increased 2–3 fold in 16 mM D-glucose with 0.1 mM IBMX as compared to islets incubated in 3 mM D-glucose either with or without IBMX. D-Glucose tested without or with IBMX had no effect on the biosynthesis of the 58-kDa protein. The 36-kDa protein was also detected in rat islets and rat islet tumor cells. It is suggested that this protein may represent a D-glucose and cAMP sensitive component of a glucose transport or glucose binding protein in the pancreatic β-cell.
【 授权许可】
Unknown
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