Glucose stimulates the biosynthesis of a human pancreatic islet cell protein detected by an antiserum against the human erythrocyte glucose transporter

FEBS Letters

Lernmark, Åke¹  Bækkeskov, Steinunn¹ 

[1] Hagedorn Research Laboratory, Niels Steensensvej 6, DK-2820 Gentofte, Denmark

关键词: Human erythrocyte glucose transporter;    Human pancreatic islet;    Glucose transport;    β-Cell protein;    Glucose regulation;    3-Isobutyl-1-methylxanthine;    NCS;    newborn calf serum;    HS;    human serum;    IBMX;    3-isobutyl-1-methylxanthine;    FCS;    foetal calf serum;    HEPES;    N-hydroxy-ethyl-piperazine-N-2-ethane-sulfonic acid;    PMSF;    phenylmethylsulfonyl-fluoride;    PCMBS;    p-chloromercuribenzenesulfonic acid;    SDS;    sodium dodecylsulfate;    cAMP;    cyclic adenosine 3′;    5′-monophosphate;   

DOI  :  10.1016/0014-5793(83)80570-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

An antiserum raised against the purified human erythrocyte glucose transporter specifically immunoprecipitated a major protein of M _r36 000 and a minor protein of M _r 58 000 from human pancreatic islets. The biosynthetic incorporation of [³⁵S]methionine into the 36-kDa protein increased 2–3 fold in 16 mM D-glucose with 0.1 mM IBMX as compared to islets incubated in 3 mM D-glucose either with or without IBMX. D-Glucose tested without or with IBMX had no effect on the biosynthesis of the 58-kDa protein. The 36-kDa protein was also detected in rat islets and rat islet tumor cells. It is suggested that this protein may represent a D-glucose and cAMP sensitive component of a glucose transport or glucose binding protein in the pancreatic β-cell.

【 授权许可】

Unknown