Measurements of cytoplasmic free Ca2+ concentration in human pancreatic islets and insulinoma cells

FEBS Letters

Köhler, Martin³  Rorsman, Patrik¹  Berggren, Per-Olaf³  Efendić, Suad³  Wiechel, Karl-Ludvig²  Kindmark, Hendrik³  Nilsson, Thomas³  Arkhammar, Per³ 

[1] Department of Medical Physics, Gothenburg University, Box 330 31, S-400 33 Gothenburg, Sweden;Department of Surgery, Södersjukhuset, Box 38 100, S-100 64 Stockholm, Sweden;The Rolf Luft Center for Diabetes Research, Department of Endocrinology, Karolinska Institute, Karolinska Hospital, Box 60 500, S-104 01 Stockholm, Sweden

关键词: Human pancreatic islet;    Human insulinoma;    Cytoplasmic free Ca2+ concentration;    Fura-2;   

DOI  :  10.1016/0014-5793(91)81309-V

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In human pancreatic islets an increase in the glucose concentration from 3 to 20 mM raised the free cytoplasmic Ca²⁺ concentration ([Ca²⁺]_i), an effect being reversible upon withdrawal of the sugar. Depolarization with a high concentration of K⁺ or the sulphonylurea tolbutamide also raised [Ca²⁺]_i. Addition of extracellular ATP produced a transient rapid rise in [Ca²⁺]_i. Oscillations in [Ca²⁺]_i were observed in the presence of 10 mM glucose. Insulinoma cells responded to glucose and tolbutamide with increases in [Ca²⁺]_i, whereas the sulphonamide diazoxide caused a decrease in [Ca²⁺]_i. These findings confirm previous results obtained in rodent β-cells.

【 授权许可】

Unknown