FEBS Letters | |
Measurements of cytoplasmic free Ca2+ concentration in human pancreatic islets and insulinoma cells | |
Köhler, Martin3  Rorsman, Patrik1  Berggren, Per-Olaf3  Efendić, Suad3  Wiechel, Karl-Ludvig2  Kindmark, Hendrik3  Nilsson, Thomas3  Arkhammar, Per3  | |
[1] Department of Medical Physics, Gothenburg University, Box 330 31, S-400 33 Gothenburg, Sweden;Department of Surgery, Södersjukhuset, Box 38 100, S-100 64 Stockholm, Sweden;The Rolf Luft Center for Diabetes Research, Department of Endocrinology, Karolinska Institute, Karolinska Hospital, Box 60 500, S-104 01 Stockholm, Sweden | |
关键词: Human pancreatic islet; Human insulinoma; Cytoplasmic free Ca2+ concentration; Fura-2; | |
DOI : 10.1016/0014-5793(91)81309-V | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
In human pancreatic islets an increase in the glucose concentration from 3 to 20 mM raised the free cytoplasmic Ca2+ concentration ([Ca2+]i), an effect being reversible upon withdrawal of the sugar. Depolarization with a high concentration of K+ or the sulphonylurea tolbutamide also raised [Ca2+]i. Addition of extracellular ATP produced a transient rapid rise in [Ca2+]i. Oscillations in [Ca2+]i were observed in the presence of 10 mM glucose. Insulinoma cells responded to glucose and tolbutamide with increases in [Ca2+]i, whereas the sulphonamide diazoxide caused a decrease in [Ca2+]i. These findings confirm previous results obtained in rodent β-cells.
【 授权许可】
Unknown
【 预 览 】
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