Purification and properties of a lectin from Lathyrus tingitanus seeds

FEBS Letters

Rougé, Pierre¹  Chabert, Philippe¹ 

[1] Laboratoire de Biologie Cellulaire, Faculté des Sciences Pharmaceutiques, 35 Chemin des Maraîchers, 31 062 Toulouse, France

关键词: Lectin;    Physicochemical property;    Biological property;    Lathyrus tingitanus;    Immunological cross reaction;    Lath-T;    Lathyrus tingitanus;    Con-A;    concanavalin A;    SDS;    sodium dodecyl sulfate;    Na2EDTA;    ethylene diamine tetraacetic acid disodium salt;    PBE;    0.1 M phosphate-buffered saline (pH 7.2);   

DOI  :  10.1016/0014-5793(83)80557-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A lectin has been isolated from the seeds of Lathyrus tingitanus by ammonium sulfate precipitation, affinity chromatography on Sephadex G-100 and subsequent chromatofocusing. This lectin has a relative molecular mass about 50 000 and consists of light (M _r 5000) and heavy subunits (M _r 20 000). The amino acid composition, N-terminal amino acids, carbohydrate and metal content of both the lectin and its subunits are given. This lectin is non-specific in agglutination of human erythrocytes and is inhibited by D-mannose, D-glucose and their α-methylglucosides derivatives. Antibodies against this lectin crossreact with other purified Lathyrus lectins and other lectins from species belonging to the Vicieae tribe.

【 授权许可】

Unknown