Two galactose‐specific receptors in the liver with different function

FEBS Letters

Kolb-Bachofen, Victoria²  Monsigny, Michel¹  Schlepper-Schäfer, Jutta²  Stockert, Richard J.³  Kolb, Hubert⁴  Roos, Peter H.² 

[1] Centre de Biophysique Moléculaire, Centre National de la Recherche Scientifique, Orleáns, France;Institut für Biophysik und Elektronenmikroskopie, Universität Düsseldorf, Moorenstr. 5, D-4000 Düsseldorf 1, FRG;Division of Genetic Medicine and The Liver Research Center, Albert Einstein College of Medicine, Bronx, NY 10461, USA;Diabetes-Forschungsinstitut, Universität Düsseldorf, Auf'm Hennekamp 65, D-4000 Düsseldorf 1, FRG

关键词: Hepatocyte;    Liver macrophage;    Asialoglycoprotein receptor;    Antibody;    Electron microscopy;    Ligand uptake;    ASF;    asialofetuin;    FITC;    fluorescein-isothiocyanate;    Gal;    D-galactose;    GalNAc;    N-acetyl-D-galactosamine;    Lac-BSA;    lactosylated bovine serum albumin;   

DOI  :  10.1016/0014-5793(83)80556-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In the rat liver both hepatocytes and macrophages have been shown to express on the surface lectins with similar binding specificity for galactose residues. Functionally the two lectins differ in the uptake of ligands. Whereas the hepatocytes ingest molecules and small particles (< 10 nm), the macrophages take up particles only. Antisera raised against hepatic galactose-specific receptor failed to react with the macrophage lectin but blocked ligand binding to the hepatocyte lectin. Accordingly, labeling with these antisera and fluorescent protein A occurs with hepatocytes only, indicating either a different antigenic structure or membrane localization of the two lectins.

【 授权许可】

Unknown