TIMP‐1 protein expression is stimulated by IL‐1β and IL‐6 in primary rat hepatocytes

FEBS Letters

Matern, Siegfried¹  Müllberg, Jürgen²  Roeb, Elke¹  Graeve, Lutz²  Rose-John, Stefan² 

[1] Medizinische Klinik III, Institut für Biochemie, RWTH Aachen, Pauwelsstrasse 30, 52057 Aachen, Germany;Institut für Biochemie, RWTH Aachen, Pauwelsstrasse 30, 52057 Aachen, Germany

关键词: TIMP-1;    Antibody;    IL-6;    IL-1;    Liver;    Hepatocyte;   

DOI  :  10.1016/0014-5793(94)00636-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Degradation of extracellular matrix proteins is performed by metalloproteinases which are inhibited by tissue inhibitors of metalloproteinases (TIMP). We expressed the murine TIMP-1 protein in E. coli and prepared a polyclonal antiserum against the recombinant protein. Using this antiserum we studied the biosynthesis and glycosylation of murine TIMP-1 protein in COS-7 cells transfected with a TIMP-1 expression plasmid by metabolic labeling and indirect immunofluorescence studies. In primary rat hepatocytes we show for the first time that TIMP-1 protein expression is up-regulated upon stimulation with IL-1β and IL-6. Since TIMP-1 is induced during the acute phase reaction it could possibly be involved in the pathogenesis of liver fibrosis.

【 授权许可】

Unknown