An enzyme synthesizing fructose 2,6‐bisphosphate occurs in leaves and is regulated by metabolite effectors

FEBS Letters

Cséke, Csaba¹  Buchanan, Bob B.¹ 

[1] Division of Molecular Plant Biology, Hilgard Hall, University of California, Berkeley, CA 94720, USA

关键词: Fructose 2;    6-bisphosphate;    Fructose 6-phosphate;    2-kinase;    Sucrose synthesis;    Gluconeogenesis;    Glycolysis;    Enzyme;    Regulation;    Fru-2;    6-P2;    fructose 2;    6-bisphosphate;    Fru-6-P;    2K;    fructose 6-phosphatase;    2-kinase;    PEG;    polyethyleneglycol;    PGA;    3-phosphoglycerate;    PFP;    pyrophosphate-linked phosphofructokinase (EC 2.7.1.90);   

DOI  :  10.1016/0014-5793(83)80226-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

An enzyme catalyzing the ATP and fructose 6-phosphate-dependent synthesis of fructose 2,6-bisphosphate, a regulator of glycolysis and gluconeogenesis, has been identified and partially purified from plants, specifically the cytoplasmic fraction of spinach leaf parenchyma cells. The enzyme, designated fructose 6-phosphate, 2-kinase, showed no response to a protein phosphorylation system known to inhibit the corresponding enzyme in mammalian cells, but it responded strikingly to metabolite effectors (P_i, an activator/PGA, an inhibitor) through changes in substrate affinity and maximal velocity. The observed pattern of regulation suggests a role for chloroplasts in controlling cytoplasmic carbon processing.

【 授权许可】

Unknown