Effect of electron transfer inhibitors on superoxide generation in the cytochrome bc 1 site of the mitochondrial respiratory chain

FEBS Letters

Tikhonov, Alexander N.²  Ruuge, Enno K.²  Ksenzenko, Marina¹  Khomutov, Gennady B.²  Konstantinov, Alexander A.¹ 

[1]A.N. Belozersky Laboratory of Molecular Biology and Bioorganic Chemistry, Moscow State University, Moscow 117234, USSR

[2]Faculty of Physics, Moscow State University, Moscow 117234, USSR

关键词: Electron-transfer inhibitor;    Superoxide generation;    Respiratory chain;    of mitochondria;    Cytochrome bc 1 site;    Q cycle;    center o;    Ubisemiquinone auto-oxidation;    NoHOQnO;    2-n-nonyl-4-hydroxyquinoline N-oxide;    BAL;    British Anti-Lewisite (2;    3-dimercaptopropanol);    SMP;    sub-mitochondrial particles;    SOD;    superoxide dismutase;    TTFA;    α-thenoyltrifluoroacetone;   

DOI  :  10.1016/0014-5793(83)80200-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Antimycin, 2-nonyl-4-hydroxyquinoline N-oxide and funiculosin induce O^·− ₂ generation by submitochondrial particles oxidizing succinate, whereas KCN, mucidin, myxothiazol or 2,3-dimercaptopropanol inhibit O^·− ₂ generation. Thenoyltrifluoroacetone does not induce superoxide production by itself but slightly stimulates the reaction initiated by antimycin. The results indicate that auto-oxidation of unstable ubisemiquinone formed in centre o of the Q-cycle generates most of the O^·− ₂ radicals in the cytochrome bc ₁-site of the mitochondrial respiratory chain.

【 授权许可】

Unknown