Direct measurement of oscillatory generation of superoxide anions by single phagocytes

FEBS Letters

Osada, Hiroyuki²  Tanaka, Kazuko¹  Tsuyama, Tomomi³  Iizuka, Tetsutaro¹  Isogai, Yasuhiro¹ 

[1] Laboratory of Biophysical Chemistry, Institute of Physical and Chemical Research (RIKEN), 2-1 Hirosawa, Wako-shi, Saitama 351-01, Japan;Laboratory of Antibiotics, Institute of Physical and Chemical Research (RIKEN), 2-1 Hirosawa, Wako-shi, Saitama 351-01, Japan;Department of Physics, Faculty of Science, Gakushuin University, Mejiro, Toshima-ku, Tokyo 171, Japan

关键词: Phagocytosis;    Superoxide generation;    Biological oscillation;    NADPH oxidase;    HL-60 cell;   

DOI  :  10.1016/0014-5793(96)00068-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Phagocytic cells such as neutrophils generate superoxide anions (O₂ ⁻) within phagocytic vacuoles for killing and digesting microorganisms. Here we report the simultaneous observation of morphological changes and O₂ ⁻ generation in single phagocytic cells during phagocytosis. Point stimulation of a cell by contact with an opsonized microelectrode at the cell surface induced significant deformation to engulf the electrode, and also induced the O₂ ⁻ generation which was measured by the electrode. Periodic fluctuations in the magnitude of the O₂ ⁻ generation were observed in the time course. These oscillations may be caused by metabolic regulation of the formation of NADPH, which is the substrate for the O₂ ⁻ generation.

【 授权许可】

Unknown