Enhanced fMLP‐stimulated chemotaxis in human neutrophils from individuals carrying the G protein β3 subunit 825 T‐allele

FEBS Letters

Rübben, Herbert²  Siffert, Gerlinde¹  Virchow, Sebastian¹  Siffert, Winfried¹  Ansorge, Nikolaus¹ 

[1] Institut für Pharmakologie, Universitätsklinikum Essen, Hufelandstr. 55, D-45122 Essen, Germany;Klinik für Urologie, Universitätsklinikum Essen, Hufelandstr. 55, D-45122 Essen, Germany

关键词: Hypertension;    Superoxide generation;    Signal transduction;    BSA;    bovine serum albumin;    [Ca2+]i;    intracellular free calcium concentration;    fMLP;    N-formyl-methionyl-leucyl-phenylalanine;    HBSS;    Hanks' balanced salt solution;    HPF;    high power field;    PMA;    phorbol 12-myristate 13-acetate;    PTX;    pertussis toxin;   

DOI  :  10.1016/S0014-5793(98)01110-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have recently described a C825T polymorphism in the gene encoding for the Gβ3 subunit of heterotrimeric G proteins. The 825T allele is associated with a novel splice variant (Gβ3-s) and enhanced signal transduction via pertussis toxin (PTX)-sensitive G proteins. fMLP-induced chemotaxis, but not O²⁻ generation, was increased in neutrophils with the TC/TT (EC₅₀=1.5±1.3 nM) genotypes compared to the CC genotype (EC₅₀=5.9±1.5 nM). Maximal fMLP-induced increase in [Ca²⁺]_i was significantly reduced in neutrophils from individuals with TC/TT genotype vs. CC genotype (212.9±10.1 nM vs. 146.4±24.2 nM). Gβ3-s appears to be associated with enhanced immune cell function in humans.

【 授权许可】

Unknown