G‐proteins in etiolated Avena seedlings Possible phytochrome regulation

FEBS Letters

Romero, Luis C.¹  Sommer, Debbie¹  Song, Pill-Soon¹  Gotor, Cecilia² 

[1] Institute for Cellular and Molecular Photobiology and Department of Chemistry, University of Nebraska-Lincoln, NE 68588-0304, USA;Department of Biochemistry, University of Nebraska-Lincoln, NE 68588-0304, USA

关键词: Avena sativa;    GTP-binding protein;    Photoregulation;    Signal transduction;    BSA;    bovine serum albumin;    Cab;    chlorophyll a/b-binding protein gene;    DTT;    dithiothreitol;    GTP-binding proteins or G-proteins;    guanine nucleotide binding proteins;    GDPβS;    guanosine 5′-O-(2-thiodiphosphate);    GTPγS;    guanosine 5′-O-(3-thiotriphosphate);    PBS;    phosphate buffered saline with 0.9% NaCl in 10mM sodium phosphate;    pH 7.2);    phy;    phytochrome gene;    PIPES;    piperazine-N;    N′-bis(2-ethanesulfonic acid);    PMSF;    phenylmethylsulfonyl fluoride;    SDS;    sodium dodecyl sulfate;    SSC;    saline sodium citrate;   

DOI  :  10.1016/0014-5793(91)80509-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The molecular mechanism of light signal transduction in plants mediated by the photosensor phytochrome is not well understood. The possibility that phytochrome initiates the signal transduction chain by modulating a G-protein-like receptor is examined in the present work. Etiolated Avena seedlings contain G-proteins as examined in terms of the binding of GTP as well as by cross-reaction with mammalian G-protein antibodies. The binding of GTP was regulated in vivo by red/far-red light. The possible involvement of G-proteins in the phytochrome-mediated signal transduction in etiolated Avena seedlings has been implicated from the study of the light regulated expression of the Cab and phy genes.

【 授权许可】

Unknown