Interaction between tissue transglutaminase and phospholipid vesicles

FEBS Letters

Horváth, Andrea¹  Hársfalvi, Jolán¹  Fésüs, László¹ 

[1] Department of Clinical Chemistry, University School of Medicine, Debrecen, PO Box 40., H-4012, Hungary

关键词: Transglutaminase;    Small unilamellar phospholipid vesicle;    Phase transition;    Enzyme—phospholipid recombinant;    Inhibition of activity;   

DOI  :  10.1016/0014-5793(83)80196-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A specific interaction between purified liver transglutaminase and small unilamellar phospholipid vesicles at the lipid phase transition have been revealed. The enzyme-induced perturbation of the bilayer is sufficient for phase transition release of encapsulated carboxyfluorescein from the vesicles. The size of the enzyme—phospholipid recombinants depends upon the protein—phospholipid ratio as shown on Sepharose 4B elution profile. The activity of transglutaminase inserted into the bilayer is greatly reduced. The interaction does not occur when the phospholipid vesicle are in the solid or liquid phase and it requires the structural integrity of the enzyme.

【 授权许可】

Unknown