Protein‐bound polyamines in the plasma of mice grafted with the Lewis lung carcinoma

FEBS Letters

Roch, A.M.¹  El Alaoui, S.¹  Delcros, J.G.¹  Quash, G.¹  Moulinoux, J.P.²  Thomas, V.¹ 

[1] Unité de Virologie fondamentale et appliquée, INSERM U51, UA.613 CNRS, 1 Place du Professeur Joseph Renaut, 69371 Lyon Cédex 08 France;Centre hospitalier Universitaire de Rennes, Groupe de recherche en Thérapeutique Anticancéreuse, 2, avenue du Professeur Léon Bernard, 35043 Rennes Cédex, France

关键词: Protein-bound polyamine;    Cancer;    Transglutaminase;   

DOI  :  10.1016/0014-5793(87)80912-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Protein-bound polyamines were isolated from the plasma of mice using antipolyamine antibodies covalently linked to magnetic latex spheres. Their subsequent separation by polyacrylamide gel electrophoresis (PAGE) showed that in plasma from normal mice, 3 proteins (27, 55 and 82 kDa) carrying polyamines could be visualized, whereas in mice bearing the Lewis lung carcinoma at least 8 other proteins of higher molecular mass (5 of 94, 110, 130, 145 and 160 kDa, and 3 of ⪢ 170 kDa) had bound polyamines. These protein-bound polyamines could be detected from the first week after tumour graft; they increased during the second and third week but decreased thereafter. These proteins were not bound by immunolatex spheres preincubated with spermine bound to a protein-carrier insulin. Moreover, the appearance of these protein-bound polyamines was not a consequence of the inflammatory process since in mice infected with heat-inactivated Brucella abortus, with the exception of a 65 kDa protein, polyamines were bound to the same proteins found in normal mice. In mice grafted with the Lewis lung carcinoma the concomitant decrease in transglutaminase-mediated polyamine (e.g. putrescine) binding capacity of plasma proteins provides additional evidence for the presence in vivo of polyamines already bound to plasma proteins.

【 授权许可】

Unknown