Small proline‐rich protein 1 is the major component of the cell envelope of normal human oral keratinocytes

FEBS Letters

Park, Myung Hee²  Marekov, Lyuben N.³  Steinert, Peter M.³  Kim, Soo-Youl³  Lee, Chong Heon²  Brahim, Jaime S.¹ 

[1] Clinical Research Core, NIDCR, National Institutes of Health, Bethesda, MD 20892-7252, USA;Oral and Pharyngeal Cancer Branch, NIDCR, National Institutes of Health, Bethesda, MD 20892-7252, USA;Laboratory of Skin Biology, Building 6, Room 425, NIAMS, National Institutes of Health, Bethesda, MD 20892-7252, USA

关键词: Oral keratinocyte;    Barrier function;    Transglutaminase;    Cell envelope;    Small proline-rich protein;    CE;    cell envelope;    NHGK;    normal human gingival keratinocytes;    TGase;    transglutaminase;   

DOI  :  10.1016/S0014-5793(00)01806-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Oral keratinocytes of buccal and gingival tissues undergo a terminal differentiation program to form a protective epithelial barrier as non-keratinized or parakeratinized stratified cells. We have examined the protein composition of cell envelopes (CEs) from normal human buccal and gingival tissues as well as keratinocytes from normal human gingival cells grown in culture. Biochemical and sequencing analyses reveal that the CEs contain 60–70% small proline-rich protein 1a/b (SPR1a/b), together with smaller amounts of involucrin, annexin I and several other known CE proteins. The data imply a specialized role for SPR1 proteins in the unique barrier function requirements of oral epithelia.

【 授权许可】

Unknown