Adenylate cyclase in cilia from Paramecium

FEBS Letters

Schultz, Joachim E.¹  Klumpp, Susanne¹ 

[1] Pharmazeutisches Institut der Universität, Morgenstelle 8, 7400 Tübingen, FRG

关键词: Adenylate cyclase;    Guanylate cyclase;    Excitable membrane;    Cilia;    Paramecium;   

DOI  :  10.1016/0014-5793(83)80180-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A particulate adenylate cyclase was identified in the excitable ciliary membrane from Paramecium tetraurelia. MnATP was preferentially used as substrate, the K _m was 67 μM, V _max was 1 nmol cAMP.min⁻¹.mg⁻¹, a marked temperature optimum of 37°C was observed. Adenylate cyclase was not inhibited by 100 μM EGTA or 100 μM La³⁺, whereas under these conditions guanylate cyclase activity was abolished. Fractionation of ciliary membrane vesicles by a Percoll density gradient yielded two vesicle populations with adenylate cyclase activity. In contrast, calmodulin/Ca-dependent guanylate cyclase was associated with vesicles of high buoyant density only.

【 授权许可】

Unknown