Characterization of sulphydryl groups of the mitochondrial phosphate translocator by a maleimide spin label

FEBS Letters

Houstek, J.³  Pavelka, S.³  Bertoli, E.¹  Megli, f.M.²  Stipani, I.²  Palmieri, F.² 

[1] Institute of Biological Chemistry, University of Bologna, Bologna 40126 Italy;Institute of Biochemistry, Faculty of Pharmacy and CNR Unit for the Study of Mitochondria and Bioenergetics, University of Bari, Bari 70126, Italy;Institute of Physiology, Czechoslovak Academy of Sciences, Prague 142 20, Czechoslovakia

关键词: Phosphate transport;    Mitochondria;    Sulphydryl group;    Maleimide spin label;    BSA;    bovine serum albumin;    NEM;    N-ethylmaleimide;    MSL;    maleimide spin label;    SDS;    sodium dodecylsulfate;   

DOI  :  10.1016/0014-5793(83)80900-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A maleimide spin label strongly inhibits the phosphate/H⁺ symporter of rat liver mitochondria. While inducing half-maximal inhibition of transport, the spin label reacts preferentially with the SH groups of the carrier, which are at least of two types. One type of SH group is localized close to the surface of the membrane and its environment does not significantly influence the mobility of the probe. The second type of SH group is buried in the membrane, is not accessible to ascorbate or chromium oxalate and its environment greatly restricts the motion of the probe.

【 授权许可】

Unknown