Z‐DNA and other non‐B‐DNA structures are reversed to B‐DNA by interaction with netropsin

FEBS Letters

Guschlbauer, Wilhelm²  Zimmer, Christoph¹  Marck, Christian² 

[1] Akademie der Wissenschaften der DDR, Zentralinstitut für Mikrobiologie und experimentelle Therapie, Abtlg. Molekulare Biochemie, Beutenbergstrasse 11, DDR-69 Jena, GDR;Service de Biochimie, Département de Biologie, Centre d'Etudes Nucléaires de Saclay, F-91191 Gif-sur-Yvette Cedex, France

关键词: Z-DNA—B-DNA reversal;    Netropsin;    Distamycin-3;    Antibiotic;    small groove-binding;    Purine—pyrimidine alternation;    Polydeoxynucleotide—antibiotic pair;    DNA conformation;    CD;    circular dichroism;    Nt;    netropsin;    Dst-3;    distamycin-3;   

DOI  :  10.1016/0014-5793(83)80894-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The interaction between the B-form specific ligands netropsin (Nt) and distamycin-3 (Dst-3) and DNA duplexes has been studied under conditions of salt concentration and low water activity that modify the polymer conformation into a non-B DNA form, putatively a Z-like form. Three polymers with strict alternating purine-pyrimidine sequences and GC content from 100—0% have been tested: poly(dG—dC)·poly(dG—dC), poly(dA—dC)·poly(dG—dT) and poly(dA—dT)·poly(dA—dT). The titrations by Nt and Dst-3 were followed by circular dichroism. Although specific binding of Nt to the Z-form of poly(dG—dC)·poly(dG—dC) does not occur, Nt reverses this Z structure to the B-type conformation; Dst-3 is, however, totally inefficient. The presumed non-B or Z-like structure of poly(dA—dC)·poly(dG—dT) is reversed to the B-form upon interaction with Nt; Dst-3 also induces this reversal but at higher ligand ratios. The modified B-structure of poly(dA—dT)·poly(dA—dT) in low water activity is efficiently reversed to the B-form by interaction with both Nt and Dst-3.

【 授权许可】

Unknown