Hydroxyl radical footprinting of the sequence‐selective binding of netropsin and distamycin to DNA

FEBS Letters

Waring, M.J.¹  Portugal, J.¹ 

[1] University of Cambridge Department of Pharmacology, Medical School, Hills Road, Cambridge CB2 2QD, England

关键词: Antibiotic-DNA binding;    Hydroxyl radical footprinting;    Iron (II)·EDTA complex;    Netropsin;    Distamycin A;   

DOI  :  10.1016/0014-5793(87)81156-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Hydroxyl radicals, generated by allowing an iron (II)·EDTA complex to react with hydrogen peroxide, have been employed to cleave the 160-base pair tyrT DNA fragment in the presence and absence of the minor groove-binding antibiotics netropsin and distamycin A. The control DNA cleavage pattern is practically independent of nucleotide sequence, which overcomes certain limitations of other footprinting techniques, so that additional information can be gained about the AT-rich sequence preference of the minor groove-binding ligands.

【 授权许可】

Unknown