Isolation and characterization of 15‐hydroxylated metabolites of leukotriene C4

FEBS Letters

Hammarström, Sven¹  Örning, Lars¹ 

[1] Department of Physiological Chemistry, Karolinska Institutet, S-104 01 Stockholm, Sweden

关键词: 15-Hydroxy leukotriene;    SRS-A;    Lipoxygenase product;    HPLC;    Guinea pig ileum bioassay;    UV spectroscopy;    HPLC;    high performance liquid chromatography;    15-HETE;    15-hydroxy eicosatetraenoic acid;    UV;    ultraviolet;   

DOI  :  10.1016/0014-5793(83)80618-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A polar metabolite of leukotriene C₄ was formed by sequential conversions with soybean lipoxygenase I and liver peroxidase. The structure of this product was found to be 5(S), 15(S)-dihydroxy-6(R)-S-glutathionyl-7,9,13-trans-11-cis-eicosatetraenoic acid (15-hydroxy-Δ¹³-trans-leukotriene C₃). The HPLC behaviour, the molar extinction coefficient and the biological activity of the metabolite are reported. Preliminary evidence suggests that this product is formed by mammalian tissues.

【 授权许可】

Unknown