Malignant hyperthermia in pigs: A search for abnormalities in Ca2+ binding proteins

FEBS Letters

Lorkin, P.A.¹  Lehmann, H.¹ 

[1] Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1QW, England

关键词: Muscular hyperthermia;    Pig troponin C;    Tryptic peptide mapping;    Calmodulin;    MH;    malignant hyperthermia;    Tn;    troponin;    2-D;    two-dimensional;    DEAE;    diethylaminoethyl;    DTT;    dithiothreitol;    EGTA;    ethyleneglycolbis (aminoethylether) tetra-acetic acid;    SDS;    sodium dodecyl sulphate;    IEF;    isoelectric focusing;    A;    absorbance;    UV;    ultraviolet;    M r;    relative molecular mass;    T;    tryptic peptide;    R;    electrophoretic mobility;   

DOI  :  10.1016/0014-5793(83)80123-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Malignant hyperthermia occurs in man and pigs as a hereditary disorder notably as a complication of halotane-induced anaesthesia. It involves an abnormality in the metabolism of Ca²⁺. A search was made for abnormalities of calcium-binding proteins. Troponin C from normal pig muscle was found to differ in 2 of 159 amino acids from rabbit Tn C and 3 from man. No differences between normal and abnormal pig muscle were found. Two-dimensional electrophoresis of red cell calmodulin from normal and abnormal pigs also failed to demonstrate a difference.

【 授权许可】

Unknown