Experimental evidence against the applicability of the Saffman‐Delbrück model to the translational diffusion of lipids in phosphatidylcholine bilayer membranes

FEBS Letters

Dieter, Hallmann¹  Vaz, Winchil L.C.¹ 

[1] Max-Planck-Institut für Biophysikalische Chemie, D-3400 Göttingen, FRG

关键词: Lipid diffusion;    Phosphatidylcholine;    Fluorescence recovery after photobleaching;    Model membrane;   

DOI  :  10.1016/0014-5793(83)80397-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The translational diffusion coefficient (D _t) of the fluorescent lipid derivative, N-(4-nitrobenz-2-oxa-1,3-diazole)-phosphatidylethanolamine (NBD-PE), with acyl chains of 6, 12, and 18 carbon atoms was measured in multibilayers of 1-palmitoyl-2-oleoyl-, dilauroyl-, and distearoyl-phosphatidylcholines using the fluorescence recovery after photobleaching technique. In a given bilayer, in the liquid crystalline phase, D _t was found to be independent of the height (length in a direction parallel to the bilayer normal) of the diffusing species. This is in disagreement with fluid hydrodynamic models for diffusion in bilayers which predict an almost linear dependence of D _t upon the height of the diffusing species in the bilayer.

【 授权许可】

Unknown