Conformational change of mastoparan from wasp venom on binding with phospholipid membrane

FEBS Letters

Takemitsu, Mari³  Nakajima, Terumi¹  Miyazawa, Tatsuo³  Higashijima, Tsutomu³  Wakamatsu, Kaori³  Fujino, Masahiko² 

[1] Institute for Medical and Dental Engineering, Tokyo Medical and Dental University, Chiyoda-ku, Tokyo 101, Japan;Chemistry Laboratories, Central Research Division, Takeda Chemical Industries, Yodogawa-ku, Osaka 523, Japan;Department of Biophysics and Biochemistry, Faculty of Science, University of Tokyo, Bunkyo-ku, Tokyo 113, Japan

关键词: Circular dichroism;    Conformational change;    1H-NMR;    Mastoparan;    Peptide-phospholipid interaction;    Venom peptide;   

DOI  :  10.1016/0014-5793(83)80385-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The conformational change upon binding with phospholipid membrane has been studied of mastoparan from wasp venom, a tetradecapeptide causing the degranulation of mast cells. The 270-MHz ¹H-NMR spectra and CD spectra indicate that the mastoparan molecule takes the α-helical conformation in methanol solution, but a much less ordered form in aqueous solution. On binding with phospholipid membrane, the α-helical conformation is formed even in aqueous medium. Such a conformational change is primarily due to the interaction between the aliphatic side chains of mastoparan and the hydrophobic interior of phospholipid membrane, in contrast to the case of melittin from bee venom.

【 授权许可】

Unknown