Activation of nucleoside diphosphate kinase by mastoparan, a peptide isolated from wasp venom

FEBS Letters

Takahashi, Koh-ichi¹  Kimura, Narimichi²  Kikkawa, Satoshi¹  Shimada, Nobuko²  Ui, Michio³  Katada, Toshiaki¹  Takahashi, Katsunobu¹ 

[1] Department of Life Science, Tokyo Institute of Technology, Yokohama 227, Japan;Department of Molecular Biology, Tokyo Metropolitan Institute of Gerontology, Tokyo 173, Japan;Department of Physiological Chemistry, Faculty of Pharmaceutical Science, University of Tokyo, Tokyo 113, Japan

关键词: GTP-binding protein. Nucleoside diphosphate kinase;    Mastoparan;    G proteins;    GTP-binding proteins;    ATP/S;    adenosine 5′-(3-O-thio)triphosphate;    GTPγS;    guanosine 5′-(3-O-thio)triphosphate;    DTT;    dithiotreitol;    SDS;    sodium dodecyl sulfate;    CHAPS;    3-[(3-cholamidopropyl)dimethyl-ammonio]-1-propanesulfonate;   

DOI  :  10.1016/0014-5793(92)80676-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have previously reported that GDP-bound αβγ-trimeric GTP-binding (G) proteins can be converted into the active GTP-bound form with nucleoside diphosphate (NDP) kinase and ATP, although its exact activation mechanism still remains to be resolved. In the present study, we investigated whether NDP kinase activity was modified by mastoparan, a wasp venom peptide that is known to activate G proteins as an agonist-receptor complex. The activity of NDP kinase measured by the formation of GTP from ATP and GDP was markedly stimulated, when the kinase was incubated with mastoparan. The concentration of mastoparan required for the activation was much lower than that observed for the peptidc-induced activation of G proteins under similar assay conditions. There was also an increase in the phosphorylated intermediate of NDP kinase as well as the catalytic activity upon its incubation with mastoparan. These results suggest that mastoparan not only activates G proteins directly via guanine nucleotide exchange reaction but also stimulates NDP kinase activity.

【 授权许可】

Unknown