Degradation of short‐lived proteins is decreased by centrifugation

FEBS Letters

Knecht, E.¹  Hernández-Yago, J.¹  Grisolía, S.¹ 

[1] Instituto de Investigaciones Citológicas de la Caja de Ahorros de Valencia, Amadeo de Saboya 4, Valencia-10, Spain

关键词: Centrifugation;    Cytochalasin B;    Enucleation;    HeLa cell;    mRNA;    Proteolysis;   

DOI  :  10.1016/0014-5793(82)80792-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have examined the effects of enucleation and of inhibitors of mRNA synthesis (actinomycin D and cordycepin) on protein turnover of HeLa cells. Enucleation markedly inhibited the rate of protein degradation for short-lived proteins. However, cells centrifuged in the absence of cytochalasin B at the speed required to obtain cytoplasts showed protein degradation rates identical to those of cytoplasts, while inhibitors of mRNA synthesis did not affect the process. Although enucleation may affect degradation of specific proteins, these results suggest that centrifugation is largely responsible for the inhibition of protein degradation in cytoplasts.

【 授权许可】

Unknown