FEBS Letters | |
Glucose transporters in chromaffin cells: Subcellular distribution and characterization | |
Torres, M.1  Delicado, E.G.1  Miras-Portugal, M.T.1  | |
[1] Departamento de Bioquímica, Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid, Spain | |
关键词: Glucose transporter; Chromaffin cell; Photoaffinity labeling; Cytochalasin B; | |
DOI : 10.1016/0014-5793(88)80792-0 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The glucose transporter was identified and characterized by cytochalasin B binding in subcellular membrane fractions of chromaffin tissue. The binding was saturable with a K d of about 0.3 μM for each subcellular fraction. The B max capacity was 12–16 pmol/mg protein for enriched plasma membrane fractions, 6.3 pmol/mg protein for microsomal membrane preparations and 5.4 pmol/mg protein for chromaffin granule membranes. Irreversible photoaffinity labelling of the glucose-protectable binding sites with [3H]cytochalasin B followed by solubilization and polyacrylamide gel electrophoresis from enriched plasma membrane preparations demonstrated the presence of three molecular species: 97±10, 51.5±6 and 30±4 kDa. The chromaffin granule membranes showed only a molecular species of 80±10 kDa.
【 授权许可】
Unknown
【 预 览 】
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RO201912020290284ZK.pdf | 295KB | download |