Glucose transporters in chromaffin cells: Subcellular distribution and characterization

FEBS Letters

Torres, M.¹  Delicado, E.G.¹  Miras-Portugal, M.T.¹ 

[1] Departamento de Bioquímica, Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid, Spain

关键词: Glucose transporter;    Chromaffin cell;    Photoaffinity labeling;    Cytochalasin B;   

DOI  :  10.1016/0014-5793(88)80792-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The glucose transporter was identified and characterized by cytochalasin B binding in subcellular membrane fractions of chromaffin tissue. The binding was saturable with a K _d of about 0.3 μM for each subcellular fraction. The B _max capacity was 12–16 pmol/mg protein for enriched plasma membrane fractions, 6.3 pmol/mg protein for microsomal membrane preparations and 5.4 pmol/mg protein for chromaffin granule membranes. Irreversible photoaffinity labelling of the glucose-protectable binding sites with [³H]cytochalasin B followed by solubilization and polyacrylamide gel electrophoresis from enriched plasma membrane preparations demonstrated the presence of three molecular species: 97±10, 51.5±6 and 30±4 kDa. The chromaffin granule membranes showed only a molecular species of 80±10 kDa.

【 授权许可】

Unknown