A protein essential for recovering oxygen evolution in cholate‐treated chloroplasts

FEBS Letters

Toyoshima, Yoshinori¹  Fukutaka, Eitaro¹ 

[1] Faculty of Integrated Arts and Sciences, Hiroshima University, 1-1-89, Higashisendamachi, Naka-ku, Hiroshima 730, Japan

关键词: Pigment system 2;    Reconstitution of oxygen evolution;    Reconstituted membrane;    Spinach chloroplast;    Thylakoid membrane;    Vesicle;    DPC;    1;    5-diphenylcarbazide;    DCIP;    2;    6-dichlorophenol indophenol;   

DOI  :  10.1016/0014-5793(82)81339-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A novel method for the reconstitution of oxygen evolution in cholate-extracted spinach thylakoid membranes was established and a protein essential for the reconstitution was purified from cholate extracts. Purification of the protein was accomplished by chromatography on a DEAE-Sephacel column. This protein (M _r 17 000) was reinserted into vesicular membranes reconstituted from cholate-extracted thylakoids in the presence of 25% glycerol to reactivate oxygen evolution.

【 授权许可】

Unknown