A 5 kDa protein (SCS23) from the 30 S subunit of the spinach chloroplast ribosome

FEBS Letters

Tanaka, Ayumi²  Koyama, Kazunari²  Maki, Yasushi²  Tsuji, Hideo²  Shimoi, Yukihiro¹  Wada, Akira¹ 

[1] Department of Physics, Faculty of Science, Kyoto University, Sakyo-ku, Kyoto 606-01, Japan;Department of Botany, Faculty of Science, Kyoto University, Sakyo-ku, Kyoto 606-01, Japan

关键词: Chloroplast ribosome;    Ribosomal protein;    Spinach chloroplast;    ID;    integrated density;    K-W;    Kaltschmidt and Wittmann's;    MW;    molecular weight;    q;    net charge;    PAGE;    polyacrylamide gel electrophoresis;    RFHR;    radical-free and highly reducing;    r-protein;    ribosomal protein;   

DOI  :  10.1016/0014-5793(93)80048-Y

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The proteins of the 30 S ribosomal subunits from spinach chloroplasts were investigated using a radical-free and highly reducing (RFHR) method of two-dimensional polyacrylamide gel electrophoresis (PAGE). Twenty-three proteins were resolved on the gel down to the smallest protein of 5 kDa. The N-terminal amino acid sequence of the 5 kDa protein showed no homology with that of any other protein stored in databases, and the copy numbers were estimated to be 0.88±0.16 and 0.72±0.04 in the 30 S subunits and the 70 S ribosomes, respectively. The results suggest that the 5 kDa protein, which we have called SCS23, may be an essential ribosomal protein specific to spinach chloroplasts.

【 授权许可】

Unknown