Evidence that a chlorophyll a' dimer constitutes the photochemical reaction centre 1 (P700) in photosynthetic apparatus

FEBS Letters

Kobayashi, Masami¹  Hiyama, Tetsuo²  Hongu, Akinori¹  Watanabe, Tadashi⁴  Murata, Norio³  Nakazato, Masataka⁵ 

[1] Department of Synthetic Chemistry, Faculty of Engineering, University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113, Japan;Department of Biochemistry, Faculty of Science, Saitama University, Urawa 338 Japan;National Institute for Basic Biology, Myodaiji, Okazaki 444, Japan;Institute of Industrial Science, University of Tokyo, Roppongi, Minato-ku, Tokyo 106, Japan;Research and Development Division, Nampo Pharmaceutical Co., Ltd, Nihonbashi-honcho, Chuo-ku, Tokyo 103,Japan

关键词: P700;    Chlorophyll a'dimer;    Photochemical reaction center;    Spinach chloroplast;    Photosynthetic pigment HPLC analysis;   

DOI  :  10.1016/0014-5793(85)80019-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Spinach chloroplasts and their various fractions enriched in either photochemical reaction centre 1 (P700) or reaction centre 2 (P680) have been analyzed for pigment composition by high-performance liquid chromatography (HPLC). The results clearly establish a stoichiometry of chlorophyll (Chl) a'/P700 = 2, where Chl a' represents the C 10 epimer of Chl a. This is the first demonstration of the association of Chl a' with any functional site in vivo. It is further shown that a Chl a' dimer prepared in vitro reproduces fairly well the redox and spectroscopic behaviour of P700. These findings provide strong evidence that a Chl a dimer constitutes P700.

【 授权许可】

Unknown