In vivo actions of protein phosphatase inhibitor‐2 in Xenopus oocytes

FEBS Letters

Foulkes, J.Gordon¹  Maller, James L.¹ 

[1] Department of Pharmacology, University of Colorado School of Medicine, 4200 East 9th Avenue, Box C236, Denver, CO 80262, USA

关键词: Microinjection;    Protein phosphatase-1;    Inhibitor-2;    Xenopus oocyte;    Oocyte maturation;    Progesterone;   

DOI  :  10.1016/0014-5793(82)81325-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Meiotic maturation of amphibian oocytes induced by progesterone is known to be regulated by protein phosphorylation. To investigate a possible role for protein phosphatase-1 in this process, the effect of phosphatase inhibitor-2 was determined on the in vivo rate of dephosphorylation of phosphorylase a and on the rate of oocyte maturation. Dephosphorylation of microinjected phosphorylase a was inhibited up to 40% in the presence of inhibitor-2, with half-maximal inhibition at an intracellular concentration of 0.6 μM. Inhibitor-2 also caused over a 3-fold increase in the half-time for maturation, suggesting a possible role for protein phosphatase-1 in the regulation of meiosis.

【 授权许可】

Unknown