Involvement of calcium, calmodulin and phospholipase A in the alteration of membrane dynamics and superoxide production of human neutrophils stimulated by phorbol myristate acetate

FEBS Letters

Christoph, Richter¹  Stocker, Roland¹ 

[1] Eidgenössische Technische Hochschule, Laboratorium für Biochemie I, Universitätstrasse 16, 8092 Zürich, Switzerland

关键词: Membrane dynamics;    Order parameter;    Neutrophil;    Calcium;    Calmodulin;    Phospholipase A;    DPH;    1;    6-diphenyl-1;    3;    5;    -hexatriene;    EGTA;    ethylene glycol-bis (β-amino ethylether-N;    N'-tetraacetic acid;    PMA;    phorbol-12-myristate-13-acetate;    PMN;    polymorphonuclear leucocytes;   

DOI  :  10.1016/0014-5793(82)81051-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have reported an increased fluorescence polarization of 1,6-diphenyl-1,3,5-hexatriene in the phorbol myristate acetate-stimulated plasma membrane of human neutrophils [FEBS Lett. (1982) 144, 199–203]. We now present evidence that both the increased fluorescence polarization and the production of super-oxide radicals by human neutrophils require calcium, calmodulin and phospholipase activity.

【 授权许可】

Unknown