Black blood T1rho MR imaging may diagnose early stage liver fibrosis: a proof-of-principle study with rat biliary duct ligation model

Quantitative Imaging in Medicine and Surgery

Chi-Man Koon¹  Eagle Siu Hong Chu²  Clara Bik San Lau³  Xin Zhang¹  Yì-Xiáng J. Wáng⁴  Weitian Chen⁴ 

[1] Institute of Chinese Medicine, State Key Laboratory of Phytochemistry and Plant Resources in West China, the Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, China;These authors contributed equally to this work.;Institute of Digestive Disease and Department of Medicine and Therapeutics, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR, ChinaInstitute of Chinese Medicine, State Key Laboratory of Phytochemistry and Plant Resources in West China, the Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, China;Department of Imaging and Interventional Radiology, the Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR, China;

关键词: T1rho (T1ρ);    liver;    fibrosis;    bile duct obstruction;    black blood;   

DOI  :  10.21037/qims.2016.08.11

学科分类：外科医学

来源: AME Publications

【 摘 要 】

Background: To explore black blood T1rho (T1ρ) liver imaging and investigate the earliest stage when biliary duct ligation (BDL) induced liver fibrosis can be diagnosed.

Methods: MR was performed at 3 Tesla. A T1ρ prepared 2D fast spin echo (FSE) sequence with acquisition of four spin lock times (TSLs: 1, 10, 30, and 50 msec) and spin-lock frequency of 500 Hz was applied. Inherent black blood effect of FSE and double inversion recovery (DIR) achieved blood signal suppression, and 3 axial sections per liver were obtained. Male Sprague-Dawley rats were scanned at baseline (n=32), and on day-3 (n=13), day-5 (n=11), day-7 (n=10), day-10 (n=4) respectively after BDL. Hematoxylin-eosin (HE) and picrosirius red staining liver histology was obtained at these time points.

Results: The physiological liver parenchyma T1ρ was 38.38±1.53 msec (range, 36.05�?41.53 msec). Liver T1ρ value elevated progressively after BDL. On day-10 after BDL all experimental animals can be separated from normal liver based on T1ρ measurement with lowest value being 42.82 msec. Day-7 and day-10 liver resembled METAVIR stage-F1/F2 fibrosis, and fibrous area counted for 0.22%±0.13% and 0.38%±0.44% of liver parenchyma area, respectively.

Conclusions: This study provides the first proof-of-principle that T1ρ might diagnose early stage liver fibrosis.

【 授权许可】

Unknown