Selective Extraction and Quantification of Glutathione using Maleimide-Presenting Gold Nanoparticles

Bulletin of the Korean chemical society

Hongseok Oh¹  Woon-Seok Yeo¹  Jeongwook Lee¹ 

关键词: Glutathione;    Quantification;    Gold Nanoparticles;    MALDI-TOF MS;    Internal standard;   

DOI  :  

学科分类：化学（综合）

来源: Korean Chemical Society

【 摘 要 】

In this paper, we describe a new method for the selective extraction and quantification of glutathione (GSH) using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and maleimide-presenting gold nanoparticles (Mal-AuNPs). Our strategy utilizes the Michael addition to selectively extract GSH, from chosen samples, onto the maleimide of Mal-AuNPs. After the extraction step, the GSH bound to the AuNPs was analyzed by MALDI-TOF MS in the presence of an internal standard which was prepared by reacting Mal-AuNPs with isotope-labeled GSH (GSH*). The GSH* has the same structure as GSH but a higher molecular weight, and therefore, enables absolute quantification of GSH by comparing the mass signal intensities of the GSH- and GSH*-conjugated alkanethiols. Our strategy was verified by analyzing GSHspiked fetal bovine serum and NIH 3T3 cells.

【 授权许可】

Unknown