Photochemical Synthesis of the Bioconjugate Folic Acid-Gold Nanoparticles

Nanomaterials and Nanotechnology

John Castillo¹  Fernando Martínez¹  Edgar Páez-Mozo¹  Linda Bertel¹ 

关键词: Photochemical Synthesis;    Gold Nanoparticles;    Folic Acid;   

DOI  :  10.5772/57144

来源: InTech

【 摘 要 】

In this paper we present a rapid and simple one-pot method to obtain gold nanoparticles functionalized with folic acid using a photochemistry method. The bioconjugate folic acid-gold nanoparticle was generated in one step using a photo-reduction method, mixing hydrogen tetrachloroaurate with folic acid in different ratios and varying the illumination time of a mercury lamp (λ = 255 nm). Scanning electron microscopy showed a particle size of around 40–50nm and dynamic light scattering exhibited that the zeta potential varies from −41 to −50mV with different illumination times. Storage in the dark at 4°C prolongs the stability of folic acid-gold nanoparticle suspensions to up to 26 days. Ultraviolet visible and Fourier transform infrared spectroscopy showed a surface plasmon band of around 534nm and fluorescence spectroscopy exhibited a quenching effect on gold nanoparticles in the fluorescence emission of folic acid and thus confirmed the conjugation of folic acid to the surface of gold nanoparticles. In t...

【 授权许可】

Unknown