Effect of pH on the Iron Autoxidation Induced DNA Cleavage

Bulletin of the Korean chemical society

Byul Nim Oh¹  Jong-Moon Kim¹  Seog. K. Kim¹  Jin-Heung Kim¹ 

关键词: Iron autoxidation;    DNA cleavage;    Fenton reaction;    Reactive oxygen species;    Hydroxyl radical;   

DOI  :  

学科分类：化学（综合）

来源: Korean Chemical Society

【 摘 要 】

Fenton reaction and iron autoxidation have been debated for the major process in ROS mediated DNA cleavage. We compared both processes on iron oxidation, DNA cleavage, and cyclic voltammetric experiment at different pHs. Both oxidation reactions were preferred at basic pH condition, unlike DNA cleavage. This indicates that iron oxidation and the following steps probably occur separately. The ROS generated from autoxidation seems to be superoxide radical since sod exerted the best inhibition on DNA cleavage when H2O2 was absent. In comparison of cyclic voltammograms of Fe2+ in NaCl solution and phosphate buffer, DNA addition to phosphate buffer induced significant change in the redox cycle of iron, indicating that iron may bind DNA as a complex with phosphate. Different pulse voltammogram in the presence of ctDNA suggest that iron ions are recyclable at acidic pH, whereas they may form an electrically stable complex with DNA at high pH condition.

【 授权许可】

Unknown