Synthesis and In vitro Evaluation of 99mTc-diglucosediethylenetriamine (DGTA) as a Potential Tumor Imaging Agent

Bulletin of the Korean chemical society

Jin Sook Ryu¹  Jung Young Kim¹  Seog-Young Kim¹  Dae Hyuk Moon¹  Sang Ju Lee¹  Seung Jun Oh¹ 

关键词: Glucose;    99mTc;    SPECT;    Tumor;    Tumor imaging;   

DOI  :  

学科分类：化学（综合）

来源: Korean Chemical Society

【 摘 要 】

Using a single step chemical synthesis, we synthesized the potential tumor imaging agent 99mTc-diglucosediethylenetriamine (DGTA) from diethylenetriamine and natural D-glucose. 10 min Incubation of 10 mg of precursor with 50 μg of SnCl2·2H2O at room temperature yielded over 95% of 99mTc labeling. The stability for 6 hours in saline or human plasma was over 90%. In vitro tumor cell uptake assays using the SNU-C5 and 9 L cell lines showed that, in 0-400 mg/dL glucose medium, cell uptake of 99mTc-DGTA was 1.5-8 times higher than that of [18F]FDG. Moreover, [18F]FDG uptake was dependent on glucose concentration in the medium, whereas cellular uptake of 99mTc-DGTA was not dependent on glucose concentration, suggesting that the two compounds have different uptake mechanisms by tumor cells.

【 授权许可】

Unknown