Bioanalytical Application of SERS Immunoassay for Detection of Prostate-Specific Antigen

Bulletin of the Korean chemical society

In Yong Eom¹  Hyeong Kuyn Seo¹  Jong Hoon Hahn¹  Young Mee Jung¹  Dongjin Pyo¹  Kyung Jin Yoon¹  Hoon Hwang¹ 

关键词: Surface-enhanced Raman scattering (SERS);    Immunoassay;    Prostate-specific antigen (PSA);    Raman reporter;    Gold nanopaticles;   

DOI  :  

学科分类：化学（综合）

来源: Korean Chemical Society

【 摘 要 】

We demonstrate the possible application of the sandwich type surface-enhanced Raman scattering (SERS) immunoassay using antigen-antibody binding for detection of prostate-specific antigen (PSA) in cancer cells. In this sandwich type of SERS immunoassay, to capture antigens onto the immobilized layer of antibodies on the gold substrate we prepared the monolayer of gold nanoparticles on the APTMS-derivatized surface of a glass slide by using the SAM technique. This sandwich type of SERS immunoassay in which antigens on the substrate specifically capture antibodies on a Raman reporter (DSNB coated gold nanoparticles with R6G) could successfully detect PSA at low levels. A strong SERS spectrum of Raman reporter was observed only with a substrate in which PSA is present.

【 授权许可】

Unknown