Micro Pre-concentration and Separation of Metal IonsUsing Microchip Column Packed with Magnetic Particles Immobilizedby Aminobenzyl Ethylenediaminetetraacetic Acid

Bulletin of the Korean chemical society

G Y Kim¹  H B Lim¹  Y H Kim¹ 

关键词: Magnetic bead;    Iron oxide;    Metal separation and concentration;    Immobilization of EDTA;    Polydimethylsiloxane microchip;   

DOI  :  

学科分类：化学（综合）

来源: Korean Chemical Society

【 摘 要 】

Magnetic beads (Dynabeads®) embedded in ~1 micron size polystyrene beads bearing surface carboxylic acid groups were modified with aminobenzyl ethylenediaminetetraacetic acid (ABEDTA) to concentrate or separate metal ions using pH gradients on micro and nano scales. The immobilization of ABEDTA was achieved by amide formation. The presence of the metal chelating functional group in the fully deprotonated form was confirmed by FT-IR. The chelation efficiency of beads was tested by determining metal ions in supernatant using GFAAS when pH gradients from 3 to 7. Mixtures of Cu and Mg and of Cd and Mn (at 10 ng/mL of metal) were separated as the difference in formation constant with the functional group of ABEDTA. The separation was repeated twice with relative standard deviation of <18%. A polydimethylsiloxane (PDMS) microchip column packed with EDTA-coated magnetic beads was optimized to concentrate metal ion for practical applications by eluting a Cu solution of micro scale at pH 3.

【 授权许可】

Unknown