Simple screening method for double-strand DNA binders using hairpin DNA-modified magnetic beads

Bulletin of the Korean chemical society

Hunho Jo¹  Kyung mi Song¹  Kyoungin Min¹  Changill Ban¹  Ja Kang Ku¹  Min su Han¹ 

关键词: Screening;    dsDNA binder;    Magnetic bead;    Hairpin DNA;    HPLC;   

DOI  :  

学科分类：化学（综合）

来源: Korean Chemical Society

【 摘 要 】

We designed an effective screening method for double strand DNA (dsDNA) binders using DNA-modified magnetic particles. Hairpin DNA was immobilized on the surface of magnetic particle for a simple screening of dsDNA binding materials in a solution containing various compounds. Through several magnetic separation and incubation processes, four DNA-binding materials, DAPI, 9AA, AQ2A, and DNR, were successfully screened from among five candidates. Efficiency of screening was demonstrated by HPLC analysis using a C2/18 reverse-phase column. In addition, their relative binding strengths were verified by measuring the melting temperature (Tm). If hairpin DNA sequence is modified for other uses, this magnetic bead-based approach can be applied as a high-throughput screening method for various functional materials such as anti-cancer drugs.

【 授权许可】

Unknown