Electrochemistry of Hemoglobin in the Chitosan and TiO2 Nanoparticles Composite Film Modified Carbon Ionic Liquid Electrode and Its Electrocatalysis

Bulletin of the Korean chemical society

Shufeng Liu¹  Wei Sun¹  Kui Jiao¹  Xiaoqing Li¹ 

关键词: Hemoglobin;    TiO2 nanoparticles;    Ionic liquid;    Carbon paste electrode;    Electrochemistry;   

DOI  :  

学科分类：化学（综合）

来源: Korean Chemical Society

【 摘 要 】

Direct electron transfer of hemoglobin (Hb) in the chitosan (CTS) and TiO₂ nanoparticles (nano-TiO₂) composite films was achieved by using a room temperature ionic liquid of 1-butyl-3-methylimidazolium hexafluorophosphate (BMIMPF₆) modified carbon paste electrode (CILE) as the basal electrode. UV-Vis and FT-IR spectroscopy indicated that Hb in the film retained the native structure. Electrochemical investigation indicated that a pair of well-defined quasi-reversible redox peaks of Hb heme Fe(III)/Fe(II) was obtained with the formal potential located at -0.340 V (ロs. SCE) in pH 7.0 phosphate buffer solution (PBS). The electrochemical parameters such as the electron transfer coefficient (�?), the electron transfer number (n) and the standard electron transfer rate constant (ks) were got as 0.422, 0.93 and 0.117 s^-1, respectively. The fabricated CTS/nano-TiO₂/Hb/CILE showed good electrocatalytic ability to the reduction of trichloroacetic acid (TCA) and hydrogen peroxide (H₂O₂), which exhibited a potential application in fabricating a new kind of third generation biosensor.

【 授权许可】

Unknown