Characterization of Czech hop (Humulus lupulus L.) genotypes by molecular methods

Plant, Soil and Environment

Patzak J.:¹ 

关键词: DNA fingerprinting;    RAPD;    STS;    ISSR;    AFLP;    genetic diversity;   

DOI  :  

学科分类：农业科学（综合）

来源: Czech Academy of Agricultural Sciences

【 摘 要 】

In the last year, new hybrid hop (Humulus lupulus L.) variety Agnus was released for cultivation in the Czech Republic. It has been necessary to prepare the quality system of Agnus identification from other Czech genotypes and characterise the germplasm of this variety by molecular methods. We proved that utilization of five STS primer combinations successfully and completely identified and determined Czech released varieties and new promising breeding materials. The use of STS method was also very effective and sensitive for control of authenticity and purity of variety Agnus in multipropagation cycle. The study of genetic diversity of 61 hop varieties by RAPD, STS, ISSR and AFLP methods confirmed, that germplasm of variety Agnus has ranked among high-alfa varieties. The results can be successfully used for identification, germplasm management, genetic studies and breeding purposes by breeders, multipropagators and hop growers.Keywords:DNA fingerprinting; RAPD; STS; ISSR; AFLP; genetic diversitydownload PDFImpact factor (Web of Science �? Thomson Reuters)2016: 1.2255-Year Impact Factor: 1.463SJR (SCImago Journal Rank �? SCOPUS)2016: 0.616 �? Q2 (Soil Science)Similarity CheckAll the submitted manuscripts are checked by the CrossRef Similarity Check.Abstracts are comprised in the databasesISI Web of KnowledgeSM, Web of Science®, Science Citation Index Expanded®, Agricola, Agrindex of AGRIS/FAO databáze, AGRIS International, CAB Abstracts, Current Contents®/Agriculture, Biology and Environmental Sciences, Czech Agricultural and Food Bibliography, Food Science and Technology Abstracts, SCOPUS, TOXLINE Plus, DOAJ (Directory of Open Access Journals), Google Scholar, CNKILicence termsAll content is made freely available for non-commercial purposes, users are allowed to copy and redistribute the material, transform, and build upon the material as long as they cite the source.Open Access PolicyThis journal provides immediate open access to its content on the principle that making research freely available to the public supports a greater global exchange of knowledge.ContactMgr. Kateřina SoučkováExecutive Editorphone: + 420 606 402 207e-mail: pseAddressPlant, Soil and EnvironmentCzech Academy of Agricultural SciencesSlezská 7, 120 00 Praha 2,Czech Republic© 2017 Czech Academy of Agricultural Sciences

【 授权许可】

Unknown