Molecular characterisation of Sporothrix schenckii isolates from humans and cats involved in the sporotrichosis epidemic in Rio de Janeiro, Brazil

Memórias do Instituto Oswaldo Cruz

Rosani Santos Reis¹  Rodrigo Almeida-paes¹  Mauro De Medeiros Muniz¹  Patrícia Morais E Silva Tavares¹  Paulo Cezar Fialho Monteiro¹  Tânia Maria Pacheco Schubach¹  Maria Clara Gutierrez-galhardo¹  Rosely Maria Zancopé-oliveira¹ 

[1] ,Fiocruz Instituto de Pesquisa Clínica Evandro Chagas Laboratório de MicologiaRio de Janeiro RJ ,Brasil

关键词: Sporotrichosis;    zoonotic transmission;    RAPD;    DNA fingerprinting;   

DOI  :  10.1590/S0074-02762009000500018

来源: SciELO

【 摘 要 】

An epidemic of sporotrichosis, a subcutaneous mycosis caused by the fungus Sporothrix schenckii, is ongoing in Rio de Janeiro, Brazil, in which cases of human infection are related to exposure to cats. In an attempt to demonstrate the zoonotic character of this epidemic using molecular methodology, we characterised by DNA-based typing methods 19 human and 25 animal S. schenckii isolates from the epidemic, as well as two control strains. To analyse the isolates, the random amplified polymorphic DNA (RAPD) technique was performed using three different primers, together with DNA fingerprinting using the minisatellite derived from the wild-type phage M13 core-sequence. The analyses generated amplicons with considerable polymorphism. Although isolates exhibited high levels of genetic relatedness, they could be clustered into 5-10 genotypes. The RAPD profiles of epidemic S. schenckii isolates could be distinguished from that of the United States isolate, displaying 20% similarity to each primer and 60% when amplified with the M13 primer. DNA fingerprinting of S. schenckii isolated from the nails (42.8%) and the oral cavities (66%) of cats were identical to related human samples, suggesting that there is a common infection source for animals and humans in this epidemic. It is clear that cats act as a vehicle for dissemination of S. schenckii.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License