Synthesis of bio-inspired Ag�?�Au nanocomposite and its anti-biofilm efficacy

Bulletin of materials science

S NEWASE²  A V BANKAR¹ 

[1] Department of Microbiology, Waghire College, Saswad, Affiliated to Savitribai Phule Pune University, Pune 412 301, India$$Department of Microbiology, Waghire College, Saswad, Affiliated to Savitribai Phule Pune University, Pune 412 301, IndiaDepartment of Microbiology, Waghire College, Saswad, Affiliated to Savitribai Phule Pune University, Pune 412 301, India$$;Department of Microbiology, Savitribai Phule Pune University, Pune 411007, India$$Department of Microbiology, Savitribai Phule Pune University, Pune 411007, IndiaDepartment of Microbiology, Savitribai Phule Pune University, Pune 411007, India$$

关键词: Nanocomposite;    biological method;    anti-biofilm;    SEM;    TEM;    XRD.;   

DOI  :  

学科分类：材料工程

来源: Indian Academy of Sciences

【 摘 要 】

In the present study, bio-inspired Ag�?�Au nanocomposite was synthesized using banana peel extract (BPE) powder. The Ag�?�Au nanocomposite was characterized using various techniques such as UV�?�vis spectrophotometry,transmission electron microscopy (TEM) attached with energy dispersive spectroscopy (EDS) and X-ray diffraction (XRD). Efficiency of AuNPs, AgNPs and Ag�?�Au nanocomposite was tested for their antibacterial activity against Pseudomonas aeruginosa NCIM 2948. The Ag�?�Au nanocomposite exhibits enhanced antimicrobial activity over its monometallic counterparts. Anti-biofilm activity of AgNPs, AuNPs and Ag�?�Au nanocomposite againstP. aeruginosa was evaluated on glass surfaces. The Ag�?�Au nanocomposite exhibited the highest biofilm reduction (70�??80%) when compared with individual AgNPs and AuNPs. Effect of AuNPs, AgNPs and Ag�?�Au nanocomposite on biofilm formation was evaluated in 96 wells microtiter plates. The percentage of biofilm inhibition was sharply increased with increasing concentration of AuNPs, AgNPs and Ag�?�Au composite. However, Au�?�Ag nanocomposite showed the highest biofilm inhibition when compared with individual AuNPs and AgNPs. This synergistic anti-biofilm activity of Ag�?�Au nanocomposite has an importance in the development of novel therapeutics against multidrug-resistant bacterial biofilm.

【 授权许可】

Unknown