期刊论文详细信息
Clinical Proteomics
Targeted quantification of N -1-(carboxymethyl) valine and N -1-(carboxyethyl) valine peptides of β-hemoglobin for better diagnostics in diabetes
Ambika G. Unnikrishnan2  Arvind M. Korwar3  Shalbha Tiwari2  Kedar B. Batkulwar1  Mahesh J. Kulkarni1  Nishita N. Meshram2  Mashanipalya G. Jagadeeshaprasad1 
[1] Proteomics Facility, Division of Biochemical Sciences, CSIR-National Chemical Laboratory, Pune, IndiaAcademy of Scientific and Innovative Research (AcSIR), New Delhi, IndiaProteomics Facility, Division of Biochemical Sciences, CSIR-National Chemical Laboratory, Pune, IndiaProteomics Facility, Division of Biochemical Sciences, CSIR-National Chemical Laboratory, Pune, IndiaAcademy of Scientific and Innovative Research (AcSIR), New Delhi, IndiaAcademy of Scientific and Innovative Research (AcSIR), New Delhi, IndiaProteomics Facility, Division of Biochemical Sciences, CSIR-National Chemical Laboratory, Pune, IndiaAcademy of Scientific and Innovative Research (AcSIR), New Delhi, India;Department of Diabetes and Endocrine Research, Chellaram Diabetes Institute, Pune, IndiaDepartment of Diabetes and Endocrine Research, Chellaram Diabetes Institute, Pune, IndiaDepartment of Diabetes and Endocrine Research, Chellaram Diabetes Institute, Pune, India;Proteomics Facility, Division of Biochemical Sciences, CSIR-National Chemical Laboratory, Pune, IndiaProteomics Facility, Division of Biochemical Sciences, CSIR-National Chemical Laboratory, Pune, IndiaProteomics Facility, Division of Biochemical Sciences, CSIR-National Chemical Laboratory, Pune, India
关键词: Diabetes;    Diagnosis;    Glycation;    Glycemic control;    HbA1c;    Mass spectrometry;   
DOI  :  10.1186/s12014-016-9108-y
来源: Humana Press Inc
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【 摘 要 】

Abstract

Background

N-1-(Deoxyfructosyl) valine (DFV) β-hemoglobin (β-Hb), commonly referred as HbA1c, is widely used diagnostic marker in diabetes, believed to provide glycemic status of preceding 90–120 days. However, the turnover of hemoglobin is about 120 days, the DFV-β-Hb, an early and reversible glycation product eventually may undergo irreversible advanced glycation modifications such as carboxymethylation or carboxyethylation. Hence quantification of N-1-(carboxymethyl) valine (CMV) and N-1-(carboxyethyl) valine (CEV) peptides of β-Hb would be useful in assessing actual glycemic status.

Results

Fragment ion library for synthetically glycated peptides of hemoglobin was generated by using high resolution–accurate mass spectrometry (HR/AM). Using parallel reaction monitoring, deoxyfructosylated, carboxymethylated and carboxyethylated peptides of hemoglobin were quantified in clinical samples from healthy control, pre-diabetes, diabetes and poorly controlled diabetes. For the first time, we report N-1-β-valine undergoes carboxyethylation and mass spectrometric quantification of CMV and CEV peptides of β-hemoglobin. Carboxymethylation was found to be the most abundant modification of N-1-β-valine. Both CMV-β-Hb and CEV-β-Hb peptides showed better correlation with severity of diabetes in terms of fasting glucose, postprandial glucose and microalbuminuria.

Conclusions

This study reports carboxymethylation as a predominant modification of N-1-β-valine of Hb, and quantification of CMV-β-Hb and CEV-β-Hb could be useful parameter for assessing the severity of diabetes.

【 授权许可】

Unknown   

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