Nonenzymatic glucosylation of proteins: a new and rapid solution for in vitro investigation

FEBS Letters

Winterhalter, Kaspar H.¹  Zimmermann, Dieter R.¹  Fischer, René W.¹  Vaughan, Lloyd¹ 

[1] Laboratorium für Biochemie, Eidgenössische Technische Hochschule, Zürich, Switzerland

关键词: Glucosylation;    Glycation;    Diabetes;    High density lipoprotein;    Low density lipoprotein;    Albumin;    PBS;    phosphate-buffered saline (pH 7.4);    HPLC;    high-performance liquid chromatography;    HDL;    high density lipoprotein;    LDL;    low density lipoprotein;   

DOI  :  10.1016/0014-5793(84)81041-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The rates of nonenzymatic glucosylation of albumin, high density lipoprotein (HDL) and low density lipoprotein (LDL) were determined in vitro using [¹⁴C]glucose repurified by a new and rapid HPLC method. All commercial preparations were found to contain contaminants reacting 15–20-times faster with protein than the repurified [¹⁴C]glucose. Removal of contaminants was critical to the rate determinations and constitutes a substantial improvement over the widely used existing method. The initial rates of nonenzymatic glucosylation determined in vitro for albumin, HDL and LDL were used to predict normal in vivo levels of 0.40, 0.65 and 0.08 mol glucose per mol protein, respectively. This is within the range of values found in vivo for albumin and LDL, but low for HDL. These values would be expected to increase 2–4-fold in diabetes.

【 授权许可】

Unknown