Urine proteome of autosomal dominant polycystic kidney disease patients

Clinical Proteomics

Agnieszka Borowiec⁴  Anna Perzanowska⁴  Stanislaw Niemczyk¹  Dominik Domanski⁴  Magda Bakun⁴  Michal Kistowski⁴  Radek Jazwiec⁴  Agnieszka Fabijanska⁴  Mariusz Niemczyk²  Leszek Paczek²  Michal Dadlez³ 

[1] Department of Internal Diseases, Nephrology and Dialysis; Military Institute of Medicine, Warsaw, PolandDepartment of Internal Diseases, Nephrology and Dialysis; Military Institute of Medicine, Warsaw, PolandDepartment of Internal Diseases, Nephrology and Dialysis; Military Institute of Medicine, Warsaw, Poland;Department of Immunology, Transplant Medicine and Internal Medicine, Medical University of Warsaw, Warsaw, PolandDepartment of Immunology, Transplant Medicine and Internal Medicine, Medical University of Warsaw, Warsaw, PolandDepartment of Immunology, Transplant Medicine and Internal Medicine, Medical University of Warsaw, Warsaw, Poland;Mass Spectrometry Laboratory, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, PolandDepartment of Biology, Warsaw University, Warsaw, PolandMass Spectrometry Laboratory, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, PolandMass Spectrometry Laboratory, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, PolandDepartment of Biology, Warsaw University, Warsaw, PolandDepartment of Biology, Warsaw University, Warsaw, PolandMass Spectrometry Laboratory, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, PolandDepartment of Biology, Warsaw University, Warsaw, Poland;Mass Spectrometry Laboratory, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, PolandMass Spectrometry Laboratory, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, PolandMass Spectrometry Laboratory, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland

关键词: ADPKD;    Urine proteome;    Differential proteomics;    Mass spectrometry;    iTRAQ;    MRM;   

DOI  :  10.1186/1559-0275-9-13

来源: Humana Press Inc

【 摘 要 】

Abstract

Background

Autosomal dominant polycystic kidney disease (ADPKD) is responsible for 10% of cases of the end stage renal disease. Early diagnosis, especially of potential fast progressors would be of benefit for efficient planning of therapy. Urine excreted proteome has become a promising field of the search for marker patterns of renal diseases including ADPKD. Up to now however, only the low molecular weight fraction of ADPKD proteomic fingerprint was studied. The aim of our study was to characterize the higher molecular weight fraction of urinary proteome of ADPKD population in comparison to healthy controls as a part of a general effort aiming at exhaustive characterization of human urine proteome in health and disease, preceding establishment of clinically useful disease marker panel.

Results

We have analyzed the protein composition of urine retentate (>10 kDa cutoff) from 30 ADPKD patients and an appropriate healthy control group by means of a gel-free relative quantitation of a set of more than 1400 proteins. We have identified an ADPKD-characteristic footprint of 155 proteins significantly up- or downrepresented in the urine of ADPKD patients. We have found changes in proteins of complement system, apolipoproteins, serpins, several growth factors in addition to known collagens and extracellular matrix components. For a subset of these proteins we have confirmed the results using an alternative analytical technique.

Conclusions

Obtained results provide basis for further characterization of pathomechanism underlying the observed differences and establishing the proteomic prognostic marker panel.

【 授权许可】

Unknown