Characterization of the Human Cervical Mucous Proteome

Clinical Proteomics

Yiming Ye¹  Gitika Panicker²  Dongxia Wang¹  Elizabeth R. Unger² 

[1] Division of Scientific Resources, National Center for Preparedness, Detection, & Control of Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, USADivision of Scientific Resources, National Center for Preparedness, Detection, & Control of Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, USADivision of Scientific Resources, National Center for Preparedness, Detection, & Control of Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, USA;Division of Viral and Rickettsial Diseases, National Center for Zoonotic, Vector-Borne, & Enteric Diseases, Centers for Disease Control and Prevention, Atlanta, USADivision of Viral and Rickettsial Diseases, National Center for Zoonotic, Vector-Borne, & Enteric Diseases, Centers for Disease Control and Prevention, Atlanta, USADivision of Viral and Rickettsial Diseases, National Center for Zoonotic, Vector-Borne, & Enteric Diseases, Centers for Disease Control and Prevention, Atlanta, USA

关键词: Cervical mucous;    Cervical cancer biomarkers;    Mass spectrometry;    2-D electrophoresis;    LC/MS/MS;    MS;   

DOI  :  10.1007/s12014-010-9042-3

来源: Humana Press Inc

【 摘 要 】

Abstract

Introduction

Cervical cancer is among the most common cancers in women worldwide. Discovery of biomarkers for the early detection of cervical cancer would improve current screening practices and reduce the burden of disease.

Objective

In this study, we report characterization of the human cervical mucous proteome as the first step towards protein biomarker discovery.

Methods

The protein composition was characterized using one- and two-dimensional gel electrophoresis, and liquid chromatography coupled with mass spectrometry. We chose to use this combination of traditional biochemical techniques and proteomics to allow a more comprehensive analysis.

Results and Conclusion

A total of 107 unique proteins were identified, with plasma proteins being most abundant. These proteins represented the major functional categories of metabolism, immune response, and cellular transport. Removal of high molecular weight abundant proteins by immunoaffinity purification did not significantly increase the number of protein spots resolved. We also analyzed phosphorylated and glycosylated proteins by fluorescent post-staining procedures. The profiling of cervical mucous proteins and their post-translational modifications can be used to further our understanding of the cervical mucous proteome.

【 授权许可】

Unknown