Microarray-based mRNA Expression Profiling of Leukemia Cells Treated with the Flavonoid, Casticin

Cancer Genomics - Proteomics

CHIARA RIGHESCHI²  ANNA RITA BILIA²  THOMAS EFFERTH¹  TOLGA EICHHORN¹  ANASTASIA KARIOTI² 

[1] epartment of Pharmaceutical Biology, Institute of Pharmacy and Biochemistry, University of Mainz, Mainz, Germanyepartment of Pharmaceutical Biology, Institute of Pharmacy and Biochemistry, University of Mainz, Mainz, Germanyepartment of Pharmaceutical Biology, Institute of Pharmacy and Biochemistry, University of Mainz, Mainz, Germany;epartment of Pharmaceutical Sciences, University of Florence, Florence, Italyepartment of Pharmaceutical Sciences, University of Florence, Florence, Italyepartment of Pharmaceutical Sciences, University of Florence, Florence, Italy

关键词: Cancer;    flavonoids;    microarrays;    multidrug resistance;    natural products;    pharmacogenomics;    CCRF-CEM;    CEM/ADR5000 cell lines;    casticin;   

DOI  :  

来源: Delinasios GJ CO

【 摘 要 】

Natural polyphenols play an important role in tumor inhibition. We used a doxorubicin-sensitive acute T-lymphoblastic leukemia cell line (CCRF-CEM) and its multidrug-resistant subline (CEM/ADR5000) to evaluate the activity of 15 plant polyphenols isolated in our laboratory (hypericin and pseudohypericin, verbascoside, ellagic acid, casticin, kaempferol-3-O-(2’’,6’’-di-E-p-coumaroyl)-glucopyranoside, kaempferol-3-O-(3,4-diacetyl-2,6-di-E-p-coumaroyl) -glucopyranoside, tiliroside, salvianolic acid B, oleuropein, rosmarinic acid, bergenin) or of others from commercial sources (curcumin, epigallocatechin-3-gallate, silymarin). Casticin was the most potent compound (IC50 values of 0.28±0.02 μM in CCRF-CEM and 0.44±0.17 μM in CEM/ADR5000 cells. The IC50 values of the other compounds tested ranged from 1.52 μM to 164.1 μM. A microarray-based mRNA expression profiling of CCRF-CEM cells treated with casticin was performed in order to identify genes with altered expression following casticin treatment. Networks related to NF-κB, p38MAPK, histones H3 and H4, and follicle stimulating hormone were identified.

【 授权许可】

Unknown