New Gene Groups Associated with Dissimilar Osteoblastic Differentiation Are Linked to Osteosarcomagenesis

Cancer Genomics - Proteomics

William R. Walsh²  Jia-Lin Yang¹  Frederick Luk²  Hai-Tao Dong²  Yan Yu² 

[1] Surgical Oncology Research Group, Department of Surgery, Prince of Wales Clinical School, Faculty of Medicine, University of New South Wales, Sydney, NSW, AustraliaSurgical Oncology Research Group, Department of Surgery, Prince of Wales Clinical School, Faculty of Medicine, University of New South Wales, Sydney, NSW, AustraliaSurgical Oncology Research Group, Department of Surgery, Prince of Wales Clinical School, Faculty of Medicine, University of New South Wales, Sydney, NSW, Australia;Surgical and Orthopedic Research Laboratories, Prince of Wales Clinical School, Faculty of Medicine, University of New South Wales, Sydney, NSW, AustraliaSurgical and Orthopedic Research Laboratories, Prince of Wales Clinical School, Faculty of Medicine, University of New South Wales, Sydney, NSW, AustraliaSurgical and Orthopedic Research Laboratories, Prince of Wales Clinical School, Faculty of Medicine, University of New South Wales, Sydney, NSW, Australia

关键词: Gene expression;    osteosarcoma;    disrupted osteoblast differentiation;    osteogenic induction;   

DOI  :  

来源: Delinasios GJ CO

【 摘 要 】

Background: Osteosarcoma has been recently redefined as a differentiation disease and its investigation is hampered by broad and complex genetic alterations. Gene expression analysis of two human osteosarcoma cell lines that are dissimilar in tumour differentiation status and osteogenic property would advance our understanding of osteo-sarcomagenesis. Materials and Methods: Gene ontology classification, hierarchical clustering, functional annotation analysis and inspection of transcription factors and their targets were used to examine differences between Saos-2 and U-2 OS cells. Microarray data were verified with real-time quantitative PCR and immunocytochemistry. Results: Genes from cell binding, cell adhesion and nervous system, as well as some well-known factors of bone formation and osteoblast characterization were identified as being differentially altered in this study. Conclusion: The osteogenicity of osteosarcoma or the disrupted osteoblast differentiation is correlated to cell binding, cell adhesion and the nervous system, as well as the osteogenic signalling system.

【 授权许可】

Unknown