Combined Elevation of microRNA-196a and microRNA-196b in Sera Predicts Unfavorable Prognosis in Patients with Osteosarcomas

International Journal of Molecular Sciences

Chun Zhang²  Cong Yao¹  Haopeng Li²  Guoyu Wang² 

[1] Department of Aesthetic Plastic Surgery, the Second Hospital Affiliated to Xi’an Jiao Tong University School of Medicine, Xi’an 710004, Shaanxi, China; E-Mail:;Department of Orthopedic Surgery, the Second Hospital Affiliated to Xi’an Jiao Tong University School of Medicine, Xi’an 710004, Shaanxi, China; E-Mails:

关键词: osteosarcoma;    microRNA-196a;    microRNA-196b;    serum;    clinicopathological features;    overall survival;    disease-free survival;   

DOI  :  10.3390/ijms15046544

来源: mdpi

【 摘 要 】

To investigate whether the aberrant expression of microRNA (miR)-196a and miR-196b can be used as potential prognostic markers of human osteosarcoma.

Quantitative real-time reverse transcriptase-polymerase chain reaction (qRT-PCR) analysis was performed to detect the expression levels of miR-196a and miR-196b in osteosarcoma tissues and patients’ sera.

Expression levels of miR-196a and miR-196b in osteosarcoma tissues were both significantly higher than those in noncancerous bone tissues (both p < 0.001), in line with which, the serum levels of the two miRNAs were also markedly upregulated in patients with osteosarcomas compared with healthy controls (both p < 0.001). Then, the elevation of serum miR-196a and miR-196b levels both more frequently occurred in osteosarcoma patients with high tumor grade (p = 0.008 and 0.01, respectively), positive metastasis (p = 0.001 and 0.006, respectively) and recurrence (p = 0.001 and 0.006, respectively). Moreover, high serum miR-196a, high serum miR-196b and conjoined expression of miR-196a/miR-196b were all independent prognostic factors for OS (overall survival) and DFS (disease-free survival) of osteosarcoma patients.

Our present data indicate the involvement of miR-196a and miR-196b upregulation in the pathogenesis of osteosarcoma. More importantly, the altered levels of circulating miR-196a and miR-196b might have great potential to serve as novel and non-invasive prognostic factors for this malignancy.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland