Diallyl Disulfide Induced Signal Transducer and Activator of Transcription 1 Expression in Human Colon Cancer Colo 205 Cells using Differential Display RT-PCR

Cancer Genomics - Proteomics

MEI-FEN TSOU⁴  HSU-FUNG LU¹  TZU-WEI TAN²  YU-CHING LI³  JAI-SING YANG⁵  SIU-WAN IP⁶  YUH-TZY LIN⁵  JING-GUNG CHUNG⁷ 

[1] epartment of Clinical Pathology, Cheng Hsin Rehabilitation Medical Center, Taipeiepartment of Clinical Pathology, Cheng Hsin Rehabilitation Medical Center, Taipeiepartment of Clinical Pathology, Cheng Hsin Rehabilitation Medical Center, Taipei;epartment of Nursing and Management, Jen-the Junior College of Medicine, Taichungepartment of Nursing and Management, Jen-the Junior College of Medicine, Taichungepartment of Nursing and Management, Jen-the Junior College of Medicine, Taichung;epartment of Medical Laboratory Science and Biotechnology, Central Taiwan University of Science and Technology, Taichungepartment of Medical Laboratory Science and Biotechnology, Central Taiwan University of Science and Technology, Taichungepartment of Medical Laboratory Science and Biotechnology, Central Taiwan University of Science and Technology, Taichung;epartment of Internal Medicine, China Medical University Hospital, Taichung, Taiwan, R.O.C.epartment of Internal Medicine, China Medical University Hospital, Taichung, Taiwan, R.O.C.epartment of Internal Medicine, China Medical University Hospital, Taichung, Taiwan, R.O.C.;epartment of Pharmacology, China Medical University, Taichungepartment of Pharmacology, China Medical University, Taichungepartment of Pharmacology, China Medical University, Taichung;epartment of Nutrition, China Medical University, Taichungepartment of Nutrition, China Medical University, Taichungepartment of Nutrition, China Medical University, Taichung;epartment of Microbiology, China Medical University, Taichungiological Science and Technology, China Medical University, Taichungepartment of Microbiology, China Medical University, Taichungepartment of Microbiology, China Medical University, Taichungiological Science and Technology, China Medical University, Taichungiological Science and Technology, China Medical University, Taichungepartment of Microbiology, China Medical University, Taichungiological Science and Technology, China Medical University, Taichung

关键词: Colon cancer cells;    diallyl disulfide;    signal transduction;    STAT1;   

DOI  :  

来源: Delinasios GJ CO

【 摘 要 】

Diallyl disulfide is one of the components of garlic and has been demonstrated to induce apoptosis in many cancer cell lines, though it is not reported to be associated with signal transducer and activator of transcription 1 (STAT1) expression. Moreover the role of STAT1 does not directly affect apoptosis in cancer cells after exposure to chemotherapy agents, though some reports showed that STAT1 is associated with apoptosis. In this study, differential display RT-PCR was used to examine the effects of diallyl disulfide (DADS) on human colon cancer cells (colo 205). The results demonstrated that DADS induced the expression of STAT1 which was also confirmed using Western blotting. STAT1 decoy oligonucleotides were also used to block STAT1 mRNA and led to a decrease in the levels of STAT1 and to subsequence decrease in the percentage of apoptosis induced by DADS in examined colo 205 cells.

【 授权许可】

Unknown